The Youth Enterprise Development Fund has made a significant contribution to the livelihood of many youths through provision of financial and supporting services especially in the rural communities in Kenya. However, questions have been raised on the efficiency of the Fund in the economic empowerment of Youth who are unemployed in Kenya. The objective of this study was to assess the effect of YEDF programme and the economic empowerment of Youth Enterprises in Matayos Sub-County in Busia County, Kenya. This study sought to finding out the effect of entrepreneurial education and training on the Youth economic empowerment in Matayos Sub County in Busia County. The sample size for the study comprised 186 Youth groups from where a sample size of 122 was derived. The study was based on the Knowledge Spillover theory. Descriptive survey research design was adopted for the study. Systematic random sampling was used to identify respondents who accessed the Youth Enterprise Development Fund, while snowball sampling was used to identify control group respondents. The analysis of the data was conducted using both descriptive statistics and inferential statistics. The findings showed that the majority of the groups had not been given entrepreneurship training. The study concluded that there was need for Business skills training before disbursement of funds in the Matayos Sub County which was an essential item in the Youth business growth and high turnover in the Sub County.